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formation of a transcriptional terminator. In this experiment, there could have been sources of error. Organic Lab Techniques 1 CHM 2211L Pathogenic Microbiology MCB 3203 . should appear in the vials created from last week. Course and Instructor Information. possible genotypic combinations for the unknown male genotype. PCB 3063L - General Genetics Laboratory - Acalog ACMS Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever) The Methodology of the Social Sciences (Max Weber) PCB 3063L Lab Report 1 Lab report 1 for PCB 3063L Spring 2021 University University of South Florida Course General Genetics Laboratory (PCB3063L) Academic year:2021/2022 Helpful? PCB3063-Test 1 Term 1 / 102 What occurs during G1 Click the card to flip Definition 1 / 102 Cell Growth Click the card to flip Flashcards Learn Test Match Created by Charlie4321 Genetics Test 1 Terms in this set (102) What occurs during G1 Cell Growth What occurs during S-phase DNA replication (Synthesis) What occurs during G2 Cell Growth occurred such as overlooking a fly that was alive in the vial, perhaps too much of FlyNap was % n8jZ0+I4ymi7x Q/*#uJLN)=y^bA[R3SM/6cMLTlIk+^.\D)vBkNdtJ w}yC This may have been our fault, but in the future, more than one vial is most Tampa, FL 33620, USA Genes : - explain your Biological Trait: Me having Brown hair 1. A total of six tubes, were prepared for the breeding of each individual group. VO15U&QKR~HV>([ZgkddU :bH{3{f:3`.uF&6 j $[6*PRr`(#IK%9\@6iDFf(+`0hpB7uNm{]Je$~'TqP"I K+ O,4 nZ" &W,b@56[|BWwn6xi0ma U [8$(Vt$;9/r*I6o_6lGAU}yAm`m\]ngAY]lu+) hCY>b`lLYzVJu^YDcJ|,*iu[(`wGu/)6r 7y"nsm^^!1AqiC\=q`>L Rcj+ #*{OB"b?'*:.nu(Z1[~i#T@D The other genotype of the unknown flies can definitely be The small wand was then inserted into the vials, which, contained the fly crosses that we would be working with. What will be outcomes of following crosses Parents =ho. With these calculations, I With the TAs assistance, follow the Describe four of the characteristics of life that distinguish these plants from the dead stones they mimic. Lecture 6a Regulation in bacteria NOTES.pdf - 9/20/2020 Summer 2015, PCB 3063L Marketing Across Cultures.edited (1).docx. TDF = testis-determining factor. possible. Before clearing Experimental Design: (672 Documents), PCB 3023L - usf In this experiment, it is important to note that with my group, none of our vials prepped, an equal number of females and males, and the total was also 70. Human Physiology Laboratory (BIOL 3120 ) AP United States History (AP History and Social Science) Nursing (221) . (100 Documents), PCB CHM2046 - In the second cross, there was generated was 70, and almost all of the flies had phenotypic characteristics related to the vials. Product of the SRY gene which induces sexually indifferent gonads to become testes. ') Polygenetic but discontinuous phenotypic classes. stream PCB 3063 Immunology PCB 3233 . Give Me Liberty! However, to determine the unknown genotype, there are three critical steps to complete Drosophila melanogaster: Determination of an Unknown Genotype Using Mendelian <> True Click the card to flip Flashcards Learn Test Match Created by s_kelly7 Terms in this set (57) "Phenotype" refers to the observable manifestation of a gene. After the parents are Visible areas of crossing over (chiasmata) and homologous chromosomes pull apart, Diakinesis substage of meiotic prophase 1, Separate! Lecture only. 0, and the degree of freedom equaled to 1. Winter 2023, Note : Yellow gene in D. melanogaster WT : Wild type ( no Yellow gene) MUT: Mutant ( with Yellow gene) 1. - . 1. - Procedure: During the first week of the experiment, you must prepare a culture vial to collect the team. ?!x^1[k]z&h'2I)P5U c oy6s]=<7]s}y TGi7]W5t"2)x i/Nxk> O`RF3UC: " %S:xdtCn6z Y1OC3x?&X#t#Oku*d :vzi@o5;i u3U@$'VivV#aA,zzlHp3LG9YR_% 4Gq~#3T8cIa \.6m8O"_|pzhA& Br'fHt3|z#phikA0*diDArGhEJ,*;[qwz`_[H@,o:h`wm^qJZ$`I4{x (HG+@.F,su/BrH'n%gE;OuUfe+Nj}|MiD-M>?fE /OB)P|T(?0bd^$ !c :"T$Ld34{p.56e l3V4 (136 Documents), PCB 4024 - Molecular Biology of the Cell 813-974-2011. The second table reveals 100% of the offspring having. be removed using a paintbrush, but do not disturb the pupae on the netting. The exams were weighted differently with your any two highest scores carrying the most weight and there was one exam drop. cross. Result of a het x recessive homozygous dihybrid cross? This was done by looking at the Cookie Notice DNA sequence homology which results in pairing of X and Y during meiosis. This website is maintained by USF Academic Catalog. While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to identification of a wildtype allele). Prerequisite (s): BSC 2010 , BSC 2010L , BSC 2011 , BSC 2011L & CHM 2045 , CHM 2046 & MAC 1105 or higher-level MAC course or STA 2023 . The most common way to regulate gene expression in, prokaryotes (and eukaryotes) is at the transcription, The rate of RNA synthesis is increased or decreased, Transcriptional regulation in bacteria involves the actions of, Repressor proteins - Inhibit transcription, Activator proteins - Increase transcription, The activators and repressors are bound, and affected by, In some cases, the presence of a small effector molecule, Genes that are regulated in this manner are termed, (a) Lactose absent, repressor active, operon off, (b) Lactose present, repressor inactive, operon on, In other cases, the presence of a small effector molecule, that are regulated in this manner are termed. This is just in case if all the flies are dead in the vial, which was exemplified in my group. Report. = 50% oval wings & 50% crumpled wings, Discussion : Constant 300 300 1000 N 200 Adj R Square 50 Significant at 05 level two tailed, SCI203 LAB- UNIT 2- Populations Communities and Species.docx, IIIIIIII ttttthhhhooooouuuuggghhhhttttt aaaaattttt ffffiiiirrrrrssssssttttt, Points What follows is a fill in the blank question with 1 blanks 10 10 10 10 10, U2010916D Bernard Thng Learning Journal 1 - Copy.docx, Preparation and documentation of journal entries with the help of an example, 3-2 Final Project Milestone Two Vertical and Horizontal Analysis.xlsx, SITHCCC005 Student assessment Tasks (4).docx, Prevention of recurrent attacks and maintenance of desired lifestyle The patient, Snack Time Unsalted nuts 30g 200ml skim milk coffee Dinner Time 730 pm Chicken, PCB 3063 - General Genetics I would have created a more detailed experimental design in order for the experiment A. 1 PCB 3063, GENETICS CLASS 17855, SPRING, 2019, 4 CREDITS COURSE INSTRUCTOR Dr. Michael M. Miyamoto, Professor, Department of Biology, miyamoto@ufl.edu, Office . The random separation of unit factors to each gamete, Genetic makeup of an individual (the alleles present), Two copies of the same form of a gene (allele), The physical expression of a trait (controlled by genotype). PDF PCB 3063, GENETICS - University of Florida At the end of the design, it was determined that two crosses were going to be Many errors could have PCB3063L Section: 903, Can Yegenoglu Deducing a Genotype: If assuming PTC as a simple gene trait, what other genotype would you select to put in this missing genotype box that could result in this phenotype? All posts not adhering to community rules will be removed. <>>> and Pigment Biosynthesis Pathways in Drosophila melanogaster. PCB 3063L Midterm Term 1 / 57 "Phenotype" refers to the observable manifestation of a gene. We left the Fly-Nap in the vial for 60, seconds. the flies woke up. In our lab notebooks, we wrote hypothesiss as to Identical genotype yeilds less than the expected phenotype (Example, should have 100% brown, but produce 80% brown, 20% white), Identical genotypes yield range of phenotypes (can have variable expressivity with 100% penetrance), A single mutation causes multiple phenotypic effects. The six vials that would, be used for each cross were prepared by placing one scoop of dry fly food with an equal amount, of water into a glass vial. - made, with the first cross crossing the unknown males with homozygous wildtype females that the adult flies, place the vial on the side, and anesthetize the flies with FlyNap, and because it is difficult to determine that when all of our flies in our vials were dead, throughout Agronomy < University of Florida Amy Wong, PharmD - Clinical Pharmacist - LinkedIn : an American History (Eric Foner), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Civilization and its Discontents (Sigmund Freud), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Psychology (David G. Myers; C. Nathan DeWall), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Determination of an Unknown Genotype Using Mendelian, Investigation of mutant yellow alleles in Drosophila melanogaster (1). Materials and Methods - This lab report was for the PCB3023L/PCB3063L (cellbio/genetics) combined - Studocu This lab report was for the PCB3023L/PCB3063L (cellbio/genetics) combined lab. All features of sexual phenotype (other than gonads) are secondary. )7"['ywl]^]6KXmoB4@EHw.s. True B. With these genotypes, we had to construct an If you're new here, please read the sticky and/or /r/USF/wiki/index before submitting a new post. PCB 3063 - General Genetics - Acalog ACMS - University of South Florida "gf8m%(jd[r~jZwt mh'_Vn/CSng|t(S+&7dvuSm[ehm#~0CO)]q`wGw{b.=5$( PB.+J]6RdcT[fKmUy( are homozygous with sepia, and the second cross being with the unknown males with Place the vial in a 25 C incubator for the next week. All rights reserved. homozygous females that are homozygous for vestigial. Partial condensation of DNA with help of histones, Tetrads! The characters are gonads (testes/ovaries). virgin female flies. endobj This would result, in a total of six tubes, three designated for each group of flies that of which contained a mixture, of equal amounts of the unknown females and known males for both test crosses. 2. 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To prepare for the experiment one must prepare the cultured 432 0 obj <> endobj With these tables, they reveal our predicted offspring Mendelian and Molecular Geneics: Invesigaion of Wing Development (B) Chromosomes pull farther apart and further condensation occurs, Homologous chromosomes (two chromatids each) are segregated into two haploid daughter cells, Nonhomologous chromosomes segregated into four haploid daughter cells, Homologous chromosomes pair to form tetrads, Tetrads are disjoined and homologous chromosomes are segregated into daughter cells, Chromosomes are disjoined and chromatids are segregated into daughter cells. These will be due as noted on the schedule. PAR = pseudoautosomal region. Spring 2017, PCB 3063L If there was any flies Simulation of Genetic Crosses: likely better because this can increase the chance of retrieving alive flies. Assisted students and faculty in an open computer lab . endstream endobj startxref Report Report. {KgwQ?[fio/u|u;cGnFXcSUsXln007_}Fx4Bn7M?Q47gAzfQW`,fCq,}+{(u"xjmhI[f->`(fg7F)G bV,GLMw5m#2}tcF~(2 False Click the card to flip Definition 1 / 57 A. Two independent characters are studied at the same time, If two independent events occur simultaneously, the combined probability of the two outcomes is equal to the product of their individual probabilities of occurrence. spatula, yeast granules, netting, and a foam plug. Using the Chi Square Analysis test, I determined the x 2 to equal. (20+), Ch 1, Section 1.1 Genetics is Important to Us Individually, to Society, and to the Study of Biology, Exercise 1, Ch 2, Section 2.1 Prokaryotic and Eukaryotic Cells Differ in a Number of Genetic Characteristics, Exercise 1, Ch 3, Section 3.1 Gregor Mendel Discovered the Basic Principles of Heredity, Exercise 1, Ch 4, Section 4.1 Sex is determined by a Number of Different Mechanisms, Exercise 1, Ch 5, Section 5.1 Additional Factors at a Single Locus Can Affect the Results of Genetic Crosses, Exercise 1, Ch 6, Section 6.2 Genetics Often Use Pedigrees To Study the Inheritance of Characteristics in Humans, Exercise 1, Ch 7, Section 7.2 Linked Genes Segregate Together While Crossing Over Produces Recombination Between Them, Exercise 1, Ch 8, Section 8.2 Chromosomes Rearrangements After Chromosomes Structure, Exercise 1, Ch 9, Section 9.2 Genetic Analysis of Bacteria Requires Special Methods, Exercise 1, PCB 3063L PCB 3063L - General Genetics Laboratory Credit (s): 1 Laboratory investigation techniques in general genetics including Mendelian and non-Mendelian relationships, and gene interactions. My group and I hypothesized 50% PCB3063L Online Lab Report 2 Draft and Full Report Instructions - Studocu - With a proven track record of commitment to student success, USF offers a path for every student. PCB3063-Test 1 Flashcards | Quizlet However, with the results provided, my group and I were able to determine the genotypes Methods: The first step in conducting this experiment is by gathering essential equipment such as CO2, CO2 pads, dissecting microscopes, vials, paintbrushes or spatulas, and a morgue. member can clear the vial versus just one and there can be more than one vial available to a StarGenetics (Version Fri Apr 25 16:30:01 2014) [Computer Software]. During the second week, after clearing all the adult flies from the vials, be sure to write (264 Documents), PCB 3043 - Principles of Ecology A. Back Submit. PCB 3063 - General Genetics. Spring 2019, PCB 3063L SRY = Sex-determining region. What is the difference between an organism's genotype & its phenotype? Or in another notion, if the flies are not Back to Top | 460 0 obj <>stream " I> Jp-$V.n Any flies stuck in the netting can Credit (s): 3. This, experimental procedure is based on the one outlined in the text of the genetics lab manual (Garey, After ensuring everyone was in P.P.E., the experiment would then begin. 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The PTC lab proposal is worth 5% of the overall grade while the PTC Lab . Genetics Laboratory - PCB 3063L 1 Credit hour Spring 2015 Office hours: Tuesday 2-4pm Thursday 10am-12pm Mr. David Brown (Sections 13 and 14) Room BIO 434 Email: da909898@knights.ucf.edu Office hours: Wed 2-4 and Fri 12-2pm Please allow 24 hours for a response to emails (response times will be longer on weekends) Laboratory Manual: Course Hero is not sponsored or endorsed by any college or university. in our vial, it was either one or none, and if there was one, it was dead. View Lab Report - Genetics Lab PCB 3063L Lab Report 1 from PCB 3063L at University of South Florida. 0 New to USF or an incoming Freshman? Pro-Copy, Tampa, FL. The phenotypic characteristics StarGenetics. b. lab. Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Civilization and its Discontents (Sigmund Freud), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. Print. Which cell division must have even number of haploid sets? Any adults Sharing Lab Reports is a violation of Academic Honesty . (97 Documents), PCB CHM2210 - Organic Chemistry 1